 |
 |
 |
|
siRNA Synthesis
Custom siRNA Synthesis
Genepharma's custom siRNA service provides the superior quality siRNAs that you need for cutting-edge gene silencing research - all at unbeatable prices. Custom siRNAs are offered at various yields and with various different modifications, including fluorescent labels. To ensure that the siRNAs are of the highest quality, all siRNAs are HPLC-purified as standard. In addition, each siRNA strand can be up to 30nt in length, allowing research on short siRNAs, long siRNAs and even dicer-substrate RNA - all at the same price.
The siRNA is provided in a stable, dried form, annealed and ready to use. The siRNA is delivered with a Technical Data Sheet that includes the name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification data. Delivery time is approximately eight to thirteen working days.
To order custom siRNA, please us the siRNA sequences together with details of any modifications or labeling. (Discounts will apply if more than one siRNA is ordered.) To help you find the perfect siRNA, Genepharma even offers a free siRNA design service - just send us the target gene ID or sequence, and we will provide you with possible siRNA sequences, selected according to the most advanced algorithms.
Standard siRNA synthesis
Standard siRNAs are unmodified and can be up to 30nt in length. The two siRNA strands are usually perfectly complementary, except for dTdT dinucleotide 3' overhangs on each strand. Other siRNA designs are, of course, also available.
|
Standard siRNA
|
|
Cat. No.
|
Yield
|
Price
|
|
A01005
|
5 OD, HPLC-purified
|
1250
|
|
A01010
|
10 OD, HPLC-purified
|
1800
|
|
A01025
|
25 OD, HPLC-purified
|
3050
|
|
A01050
|
50 OD, HPLC-purified
|
5150
|
|
A01100
|
100 OD, HPLC-purified
|
8550
|
|
A01250
|
250 OD, HPLC-purified
|
16450
|
-
1 OD is equivalent to approximately 2.5 nmoles of HPLC-purified siRNA duplexes.
Enhance siRNA Stability with Chemical Modifications
Unmodified siRNAs are susceptible to degradation by nucleases. A convenient solution to this problem involves the use of modified RNA nucleotides that confer nuclease resistance while retaining hydrogen bonding properties. Genepharma offers 2'-O-methyl- as well as 2-fluoro-modified siRNAs.
|
Chemically modified siRNA
|
|
Cat. No.
|
Yield
|
Price
|
|
A02005
|
5 OD, HPLC-purified
|
1450
|
|
A02010
|
10 OD, HPLC-purified
|
2050
|
|
A02025
|
25 OD, HPLC-purified
|
3650
|
|
A02050
|
50 OD, HPLC-purified
|
5900
|
|
A02100
|
100 OD, HPLC-purified
|
10400
|
|
A02250
|
250 OD, HPLC-purified
|
20400
|
Visualize siRNA Uptake with Fluorescent Labels
Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. Fluorescent labeling of siRNAs allows direct observation of their cellular uptake, distribution, and localization as early as 3-4 hours after transfection. In contrast, uptake of unlabeled siRNAs must be monitored by target gene knockdown. Fluorescently labeled siRNAs are also ideal for examining the correlation between transfection efficiency and gene knockdown.
Genepharma offers FAM- as well as TAMRA-labeled siRNA. The fluorescent labels are usually added to the 5' end of the sense strand, and do not affect the biological activity of the siRNA. Other labels such as DABCYL and Biotin are also available upon request.
|
Fluorescently labeled siRNA
|
|
Cat. No.
|
Yield
|
Price
|
|
A03005
|
5 OD, HPLC-purified
|
1600
|
|
A03010
|
10 OD, HPLC-purified
|
2400
|
|
A03025
|
25 OD, HPLC-purified
|
4200
|
|
A03050
|
50 OD, HPLC-purified
|
7250
|
|
A03100
|
100 OD, HPLC-purified
|
10100
|
|
A03250
|
250 OD, HPLC-purified
|
23000
|
siRNA experimental controls
In addition to the fluorescently labeled siRNA, Genepharma offers a variety of internal controls for development and optimization of siRNA experiments:
-
Negative control siRNAs are designed to have the same nucleotide composition as your siRNA, but without homology to the genome of the target organism.
-
A large range of positive control siRNAs are available, each with published and validated efficiency against the target gene.
-
In addition, to assess the extent to which the transfection procedure itself perturbs gene expression, mock transfection using only the transfection reagent is very useful.
|
Experimental controls
|
|
Cat. No.
|
Yield
|
Price
|
|
B01001
|
Negative control siRNA (1 OD, HPLC)
|
720
|
|
B02001
|
FAM-labeled negative control siRNA (1 OD, HPLC)
|
800
|
|
A03025
|
Positive control siRNA (1 OD, HPLC)
|
720
|
|
A03050
|
RNAi-mate transfection reagent
|
750
|
|
Frequently used positive control siRNA
|
|
Target gene name
|
Target gene name
|
|
ß-actin
|
GalTII (galactosyltransferase II)
|
|
gamma-actin
|
GAS41 (1)
|
|
AIB1 (estrogen receptor coactivator)
|
Hec-1
|
|
Apaf-1
|
HPV E6
|
|
ARC21
|
HPV E7
|
|
ATR interacting protein
|
Tissue Factor (hTF)
|
|
Bcr-abl
|
Vimentin
|
|
beta-tubulin- mouse neuronal
|
HtrA2
|
|
Bruton's tyrosine kinase (Btk)
|
keratin 18
|
|
Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta)
|
Kinase interacting stathmin (hKIS)
|
|
Caspase 1
|
lamin A
|
|
Caspase 2
|
lamin A/C
|
|
hCdc2
|
lamin B1
|
|
Cdc14A
|
lamin B2
|
|
cdk1
|
LAP2
|
|
CENP-E
|
Mad1
|
|
CHO1
|
Mammalian septin (MSF)
|
|
corin, mouse
|
Methyl-CpG-binding protein 2 (MeCP2)
|
|
Cyclin B1
|
Methyl-DpG binding domain protein 2 (MBD2)
|
|
Cyclin B2
|
MPS1 kinase (hMps1)
|
|
cytoplasmic dynein 1 heavy chain
|
Nicastrin (Nct)
|
|
DIP13-alpha (DCC interacting protein)
|
NuMA
|
|
Dishevelled (hDv-1 and hDv-3)
|
Nup153
|
|
hDv-2
|
P160 ROCK
|
|
Eg5
|
Polo-like Kinase1 (hPlk1)
|
|
emerin
|
Protein Kinase Receptor (PKR)
|
|
eve
|
hRad9
|
|
hFbw7 (cyclin E ubiquitination)
|
Rad17
|
|
Fortilin
|
Myeloid cell leukemia protein 1 (MCL1)
|
|
GalTII (galactosyltransferase II)
|
|
|
|
|
 |
|
 |
|
|
 |
|
|
 |
| |
 |
DNA oligo synthesis
Highest quality, lowest cost custom oligos. Cartridge purified free of charge.
|
Lab consumables
A comprehensive range of PCR products, restriction endonucleases, extraction kits and other laboratory consumables.
|
Custom services
In addition to custom oligo synthesis, Sylvean also offers siRNA, peptide and gene synthesis services - all at amazing prices.
|
|
 |
| |
 |
|
|
|
|
